Aniline hydrochloride

142-04-1

Dye intermediate; dyeing and printing.

White crystals, darkens in light and air.

129.59

Slight fire hazard when exposed to flame or hear; emits toxic
aniline and chlorine fumes when decomposed or reacted with acid. 
Soluble in alcohol, ether. 
Soluble in approximately 1 part
water.

Administration of 50 mg/kg injected ivn to rabbits had a
halflife in the blood of 295 min (n=6). -  
When added to the
perfusion fluid in amounts of 0.0002 - 0.100 mmol aniline
hydrochloride was poorly accumulated (10 - 20 %) by isolated
perfused rabbit lungs, artificially ventilated and perfused via
the pulmonary artery with heparin treated, antologous whole
blood. 
Uptake was rapid and steady-state was reached in 10
minutes. -  
Male rats were administered aniline HCl by gavage
at 3 doses: 10, 30, and 100 mg/kg. 
At selected times, rats were
sacrificed and brain, lung, kidney, liver, spleen, heart and
plasma analyzed for radioactivity. 
Urine and feces were
collected at 12 hr intervals for 48 hr after treatment.

Results: Peak plasma radioactivity was observed at 0.5, 1.0,
and 2.0 hr for 10, 30, and 100 mg/kg doses, respectively. 
By 24
hr after dosing, the plasma radioactivity had decreased to < 2 %
of peak concentration for all dosages. 
Radioactivity was
distributed to all tissues, with highest peak levels observed
in the kidney, followed by liver, plasma, lung, heart, spleen
and brain for all doses. 
By 48 hr after dosing, < 0.1 % of
total administered radioactivity at any of the three doses
remained in any of the tissues examined. 
Recovery of
radioactivity in urine 48 hr after dosing was 96, 91, and 77 % for
the 10, 30, and 100 mg doses, respectively. 
Results
indicate that aniline HCl is rapidly eliminated from the rat in
the urine, particularly as an acid-hydrolyzable metabolite of
p-aminophenol. -  
The gastrointestinal tract seems to be a
major site for the metabolism of aniline in the rat following
ivn injection of 3, 30 or 100 mg/kg to male Fischer 344 rats
(Sax 1986).

Skin and eye irritation data:
skn, rbt, 500 mg, 24 hr, moderate;
eye, rbt, 20 mg, 24 hr, severe (Sax 1986).

Dietary administration was found to be carcinogenic to male and
female Fischer 344 rats, inducing hemangiosarcomas and a
combination of fibrosarcomas and sarcomas, NOS or the spleen
and a combination of fibrosarcomas and sarcomas, NOS of
multiple body organs. 
There was no evidence of carcinogenicity
in mice of either sex (Sax 1986).

Mutagen data:
otr, rat, emb, 79500 ng/plate;
sce, ham, fbr, 0.010 mmol/L (Sax 1986).

NCI carcinogenesis bioassay completed; result positive:rat;
results negative: mouse (Sax 1986).

Fish: goldfish, approx. fatal conc. 5.5 mg/l, 48hr (McKee &
Wolf 1963).

                        effect level (mg/l)
Chironomus dorsalis     7.8
Chironomus dorsalis     5.0
Carassius auratus       5.5 (48 hr)
Cyprinus carpio         0.5 - 48.0 (90 days)
Cyprinus carpio         0.1 (90 days)        (Sax 1986).